autophagy activator rapa Search Results


autophagy activator rapa  (Solarbio Inc)
90
Solarbio Inc autophagy activator rapa
<t>RAPA</t> reversed the effects of LACTB overexpression on the proliferation, ROS production and apoptosis <t>of</t> <t>MGC-803/OXA</t> cells. After transfection with LACTB plasmid, MGC-803 cells were treated with RAPA or/and OXA, respectively. A. CCK-8 assay was carried out to assess the proliferation changes of MGC-803/OXA cells in each group. ***P < 0.001 vs. vector group; ##P < 0.001, ###P < 0.001 vs. LACTB group; &P < 0.05, &&P < 0.01 vs. LACTB+RAPA group. B. EdU staining was utilized to detect the proliferation changes of MGC-803/OXA cells in each group. C. DCFH-DA fluorescent probe was utilized to confirm the intracellular level of ROS. D. Flow cytometry was applied to analyze the apoptosis of MGC-803/OXA cells. *P < 0.05; **P < 0.01, ***P < 0.001.
Autophagy Activator Rapa, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/autophagy+activator+rapa/autophagy+activator+rapa/pmc07868839-148-11-14
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autophagy activator rapa - by Bioz Stars, 2026-10
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RAPA reversed the effects of LACTB overexpression on the proliferation, ROS production and apoptosis of MGC-803/OXA cells. After transfection with LACTB plasmid, MGC-803 cells were treated with RAPA or/and OXA, respectively. A. CCK-8 assay was carried out to assess the proliferation changes of MGC-803/OXA cells in each group. ***P < 0.001 vs. vector group; ##P < 0.001, ###P < 0.001 vs. LACTB group; &P < 0.05, &&P < 0.01 vs. LACTB+RAPA group. B. EdU staining was utilized to detect the proliferation changes of MGC-803/OXA cells in each group. C. DCFH-DA fluorescent probe was utilized to confirm the intracellular level of ROS. D. Flow cytometry was applied to analyze the apoptosis of MGC-803/OXA cells. *P < 0.05; **P < 0.01, ***P < 0.001.

Journal: American Journal of Translational Research

Article Title: LACTB induced apoptosis of oxaliplatin-resistant gastric cancer through regulating autophagy-mediated mitochondrial apoptosis pathway

doi:

Figure Lengend Snippet: RAPA reversed the effects of LACTB overexpression on the proliferation, ROS production and apoptosis of MGC-803/OXA cells. After transfection with LACTB plasmid, MGC-803 cells were treated with RAPA or/and OXA, respectively. A. CCK-8 assay was carried out to assess the proliferation changes of MGC-803/OXA cells in each group. ***P < 0.001 vs. vector group; ##P < 0.001, ###P < 0.001 vs. LACTB group; &P < 0.05, &&P < 0.01 vs. LACTB+RAPA group. B. EdU staining was utilized to detect the proliferation changes of MGC-803/OXA cells in each group. C. DCFH-DA fluorescent probe was utilized to confirm the intracellular level of ROS. D. Flow cytometry was applied to analyze the apoptosis of MGC-803/OXA cells. *P < 0.05; **P < 0.01, ***P < 0.001.

Article Snippet: Besides, cells were treated with 4 μmol OXA or 100 nM autophagy activator RAPA (Solarbio, Cat.NO.

Techniques: Over Expression, Transfection, Plasmid Preparation, CCK-8 Assay, Staining, Flow Cytometry

LACTB suppressed mitochondrial depolarization, glucose uptake and ATP synthesis, and reduced mitochondria of MGC-803/OXA cells by autophagy pathway. A. The mitochondrial depolarization was examined using flow cytometry with JC-1 staining. B. Mitochondrial damage of MGC-803/OXA cells was evaluated by TEM after treatment with LACTB plasmid, RAPA or/and OXA. C. D. Respective kits were used to determine the levels of 2-DG uptake and ATP in the treated MGC-803/OXA cells. *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: American Journal of Translational Research

Article Title: LACTB induced apoptosis of oxaliplatin-resistant gastric cancer through regulating autophagy-mediated mitochondrial apoptosis pathway

doi:

Figure Lengend Snippet: LACTB suppressed mitochondrial depolarization, glucose uptake and ATP synthesis, and reduced mitochondria of MGC-803/OXA cells by autophagy pathway. A. The mitochondrial depolarization was examined using flow cytometry with JC-1 staining. B. Mitochondrial damage of MGC-803/OXA cells was evaluated by TEM after treatment with LACTB plasmid, RAPA or/and OXA. C. D. Respective kits were used to determine the levels of 2-DG uptake and ATP in the treated MGC-803/OXA cells. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Besides, cells were treated with 4 μmol OXA or 100 nM autophagy activator RAPA (Solarbio, Cat.NO.

Techniques: Flow Cytometry, Staining, Plasmid Preparation

LACTB resulted in OXA-induced DNA damage by inhibiting autophagy pathway in MGC-803/OXA cells. A. IF assay was performed to assess the degree of LC3B. Magnification 200 ×, Scale bars = 20 μm. B. Markers of DNA double-stranded damage and autophagy were examined by Western blot assay in MGC-803/OXA cells after treatment with LACTB plasmid, RAPA or/and OXA. C-H. The relative gray density was counted by comparison with GAPDH. *P < 0.05; **P < 0.01, ***P < 0.001.

Journal: American Journal of Translational Research

Article Title: LACTB induced apoptosis of oxaliplatin-resistant gastric cancer through regulating autophagy-mediated mitochondrial apoptosis pathway

doi:

Figure Lengend Snippet: LACTB resulted in OXA-induced DNA damage by inhibiting autophagy pathway in MGC-803/OXA cells. A. IF assay was performed to assess the degree of LC3B. Magnification 200 ×, Scale bars = 20 μm. B. Markers of DNA double-stranded damage and autophagy were examined by Western blot assay in MGC-803/OXA cells after treatment with LACTB plasmid, RAPA or/and OXA. C-H. The relative gray density was counted by comparison with GAPDH. *P < 0.05; **P < 0.01, ***P < 0.001.

Article Snippet: Besides, cells were treated with 4 μmol OXA or 100 nM autophagy activator RAPA (Solarbio, Cat.NO.

Techniques: Western Blot, Plasmid Preparation